PRODUCTION, PURIFICATION AND CHARACTERIZATION OF EXTRACELLULAR L-ASPARAGINASE FROM ERWINIA CAROTOVORA

Author(s): El shobaky Ahmed*, Mohamad A Abbas and Shymaa N Askar

Abstract

L-asparaginase is an anti-neoplastic agent used in lymphoblastic leukemia chemotherapy. Bacterial isolates were screened for potential production of L-asparaginase using a phenol red indicator growth medium and the bacterium that produce largest hydrolysis zone was selected. The isolate was characterized by biochemical tests and was found to belong to Erwinia carotovora (crude Enzyme production = 176.27 U/ml and specific activity = 3.5 U/mg protein). The enzyme production was induced by different carbon and nitrogen sources, Lactose was found to be the best carbon source and ammonium nitrate was the best nitrogen source for production of the enzyme at pH 7 and 37°C. The enzyme was partially purified by ammonium sulphate precipitation and complete purification was achieved by ion-exchange chromatography (1138.28 U/ml and specific activity = 25.5 U/mg protein), followed by enzyme characterization.

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