Isolation and characterization of Swertiamarin from aerial parts of Enicostemma littorale blume

Author(s): Ravi Kumar Kakarla, Madhanraj Rajendran, Balaji Paulraj, Eyini Muthukumaraswamy

Abstract

Enicostemma littorale Blume (Fam. Gentianaceae) is commonly known as Nagajihva, is used in traditional system of medicine since long time. Many compounds have been reported from the various parts of the plant. In the present study, n-butanol soluble extract of the aerial parts of the plant was subjected to column chromatography to isolate and purify the phytoconstituents which can be termed as marker. One compound was isolated and characterized by NMR (Nuclear Magnetic Resonance), IR (Infra-red Spectroscopy), and Mass spectroscopy as Swertiamarin. Novel HPLC (High Performance Liquid Chromatography) methods were developed for assessment of purity, for standardization and for estimation of the compound. Simplicity of isolation and HPLC analysis of the compound suggests that the compound may be termed as markers for the standardization of the extracts and preparations containing Enicostemma littorale.

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