Influence of hydrogen peroxide induced oxidative stress on survival rate of early chick embryo development

Author(s): Jaywant Tanaji Jadhav*, Suryakant Kengar

Abstract

Reactive oxygen species (ROS) induced oxidative stress influences embryonic growth and development. Hydrogen peroxide (H2O2) is a major source of ROS generation that induces oxidative stress by altering redox status. In present study, direct effect of H2O2 generated oxidative stress on survival rate and associated toxicity in chick embryo was studied during early development. Chick embryos at 96 hrs of incubation were treated with single doses of 2.5μg, 5.0μg, 10μg, 50μg, 100μg, 300μg and 500μg H2O2 concentrations per embryo. The toxic effects recorded after 144 hrs of development. The results showed that treatments of 2.5 and 5.0μg H2O2 doses did not affect survival rate and embryo development. 10μg and 50μg H2O2 doses treatment exhibited slightly reduced survival rate without affecting normal morphology. Administration of 100 and 300μg H2O2 doses caused predominant decrease in the survival rate in comparison with the normal and PBS treated control embryos with deformities such as growth retardation, defected brain, limbs and vascular development with hemorrhage. Treatment of 500μg H2O2 exhibited no survival of embryos. These results indicated that post-omphalomesentric stages of early chick embryos are more susceptible to elevated level of H2O2 induced oxidative stress leading to significant reduction in survival rate with associated deformities. These results are discussed with probable reasons.

image 10.21746/ijbio.2016.06.004

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