IDENTIFICATION OF HELICOBACTER PYLORI STRAIN BY PCR IN ENDOSCOPIC SAMPLES FROM PEPTIC ULCER PATIENTS

Author(s): Santhosh V, Kavitha, GV Prameela, N Rajkumar, G Shanthi, S Sethupathy and N Prasada Rao

Abstract

Worldwide studies have shown that H. Pylori infections are strongly associated with peptic ulcer patients. Various studies have shown that there is a definitive impact on genotypic classification of H. Pylori on treatment strategy and the prevalence of H. Pylori is not homogeneous and varies depending on the country of origin, ethnic background and socio economics status. There is no reported evidence of genotype specific H. Pylori detection in our population and so the focus of the study is to classify H. Pylori detection in our population which would help in the effective treatment of H. Pylori infection. The study included 32 patients with peptic ulcer disease who were posted for gastric endoscopy in the surgery OPD. Gastric antral biopsy was collected for all the patients by endoscopy. Culture of the biopsy was done by sheep blood agar. Genotyping of H. Pylori was done by PCR. 16srRNA and Cag A genes of H. Pylori are said to be commonly associated with peptic ulcer. The study showed the prevalence of 16 srRNA in all the 32 patients, which confirms the presence of H. Pylori, but Cag A strain was negative for all cases, thus necessitating the implementation of specific genotype oriented treatment approach.

image 10.21746/ijbio.2014.08.003

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