Human Genetics Congress 2019- Effect of a three-dimensional matrix with calcium phosphate coating on the functional activity of Jurkat cells during cultivation with MMSC

Author(s): Khaziakhmatova Olga

Abstract

Introduction: Currently, traumatology actively uses ceramic materials with calcium phosphate coating (CP). Such materials have a stimulating effect on multipotent mesenchymal stromal cells (MMSCs) and immunocompetent cells. So, their use involves an assessment of the impact of these materials on the development of tumor cells. Methodology: The Jurkat 5332 cell line and human adipose-derived MMSCs (AMMSCs) were examined. 3D culture was simulated by adding to the cell culture the substrates from commercially pure titanium with rough (Ra=2-5 μm) CP microarc coating. The cultures were used: 2D with Jurkat T cells (JTCs) on plastic surface; 2D co-culture of JTCs and AMMSCs on plastics; 3D with JTCs and CP matrix; 3D with JTCs, AMMSCs, and CP matrix. Findings & Conclusion: Both 2D and 3D JTC cultures showed an increase in the CD45RO receptor expression that led to increasing number of CD45RO+CD45RA+ cells.

Probably, JTCs restored the partial maturation and differentiation. Vice versa, the expression of CD45RO receptor and the number of CD45RO+CD45RA+ cells decreased in case of JTCs and AMMSCs co-cultivation. A similar reaction of the cells was revealed in the 3D culture of JTCs and AMMSCs. Thus, JTCs with AMMSCs co-cultivation may promote the preservation of the tumor cell phenotype and survival. An additional confirmation of the differentiation of JTCs was a decrease in the number of CD25 + and CD95 + cells in the presence of matrices. Moreover, decreased content of IFN-γ and IP-10 in cell supernatants. The cultivation of AMMSC and Jurkat revealed a significant increase in the production of IFN-γ (3 times) and IP-10 (10 times). But despite this, the level of chemokine IP-10 in the presence of matrices with CP coating was significantly reduced; the content of INF-γ remained practically unchanged.

Cultivation of JTCs with matrices with CP coating resulted in a 3-fold decrease in the level of MCP-1 in culture supernatants. The cultivation of AMMSC with Jurkat in a 2D model revealed an increase in the concentration of chemokines MCP-1 (20 times) and RANTES (400 times). In the 3D cultivation model, the secretory activity of the mixed culture decreased and was similar to the level of AMMSC 3D monoculture.

Khaziakhmatova Olga, PhD, is a researcher at the Center for Immunology and Cellular Biotechnology, IKBFU. The main areas of work are cell technology and she also participates in stem cell and immunocompetent cell research projects.

Share this article

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.