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Enzyme modulation studies of fumaronitrile on fresh water fish Oreochromis mossambicus

Author(s): Chinnadurai Kuppusamy, Arul Ganesh, Karthikeyeni Sambanthan, Eyini Muthukumarasamy, Balaji Paulraj


The present study was directed to analyse the potential enzymatic variation efficiency of fumaronitrile on fresh water fish tilapia (Oreochromis mossambicus). To study the acute toxicity of xenobiotic five different concentrations (20, 40, 60, 80 and 100ppb) of fumaronitrile along with control group was maintained for over 96 hrs. At every 1 hr. fishes was monitor both control and treatment groups. The LC50 value was found to be as 60ppb. To study the chronic toxicity of xenobiotic three different concentrations (2ppb, 4ppb and 6ppb) of fumaronitrile along with control group was maintained for over 100 days. At 60, 80,100 days’ fishes were sacrificed both control and treatment groups and the organs such as liver were collected to analyse the changes in testicular enzyme activities and an increasing pattern for both antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) was observed. Enzyme assay is one of the important methods to evaluate the toxicity of xenobiotic. At present, no report is available on the effect of fumaronitrile on antioxidant enzymes and testicular enzyme activities. This study focus light on the effects of fumaronitrile in antioxidant enzymes CAT, SOD and testicular enzymes ALP, ACP, LDH and SDH in Oreochromis mossambicus.

image 10.21746/ijbio.2016.11.0011

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