Development of ELISA techniques for haemorrhagic septicaemia
Author(s): Subhash Kharb
Haemorrhagic septicaemia (HS) caused by Pasteurella multocida serotypes B:2 and E:2 in Asian and African countries respectively is a major epizootic disease of cattle and buffaloes with heavy morbidity and mortality. Being simple, rapid, inexpensive and easy for automation, ELISA has emerged as an important tool for diagnosis as well as monitoring the immune status of animals vaccinated against HS in laboratories. In the present review, development of ELISA techniques for diagnosis, sero-surveillance of immune status in vaccinated animals and DIVA strategy for the evaluation of various HS control programmes have been discussed. Among the various variants, indirect-ELISA has been found most commonly used format for sero-surveillance against HS vaccines and quantification of antibody responses in different vaccine formulation trails. The development of monoclonal antibodies based ELISA have increased the specificity and sensitivity of the test.
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