DEVELOPMENT AND VALIDATION OF LC METHOD FOR THE ESTIMATION OF LINCOMYCIN IN PHARMACEUTICAL DOSAGEFORMS

Author(s): YLN Murthy, Madhira VNS RamPrasad, P Mahesh, B Dilip Kumar1, YN Sastry and P Mani

Abstract

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Lincomycin in tablet dosage form. An Inertsil ODS C-18, 5µm column having 250 x 4.6mm internal diameter in isocratic mode with mobile phase containing methanol: Acetonitrile: 1%OPA (10:65:25v/v/v) was employed. The flow rate was 1.0ml/min and effluents were monitored at 258nm. Ornidazole was used as an internal standard. The retention time for Lincomycin and Ornidazole were 8.8min and 2.6 min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection and limit of quantification were found to be 0.05ppm and 0.15ppm respectively and recovery of Lincomycin from tablet formulation was found to be 101.307%. The proposed method was successfully applied for the quantitative determination of Lincomycin in tablet formulation.

Share this article

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.