Decolorization of some textile dyes by Pleurotus citrinopileatus and Aspergillus niger immobilized on wheat straw

Author(s): Eman HF Abd El-Zaher*, Azza A. Mostafa, Sahar FM Al- Hefnawy

Abstract

The potential of wheat straw immobilized Aspergillus niger and Pleurotus citrinopileatus were studied for biodegradation ability of different types of textile dyes. Non exposed and exposed tested immobilized fungi to (1 KGy and 2 KGy) of gamma irradiation were used. The tested textile dyes were Reactive red Bx, Direct Brown BN, Direct yellow 5G, disperse orange 2 RLS, Disperse Violet 3 RS, Sulpher Black Br and Sulpher Brown SG. It was found that immobilized P. citrinopileatus exposed to (2 KGy) had more efficiency for decolorization of direct brown and direct yellow dyes. Decolorization percentage of immobilized P. citrinopileatus in Direct Brown and Direct yellow reach to 95% and 90% respectively after 8 days at 28oC. Peroxidase activity for (1 KGy) irradiated P. citrinopileatus is more than peroxidase activity of unirradiated P. citrinopileatus. RAPD.PCR has been performed to know the effect of gamma rays on P. citrinopileatus; RAPD profile shows change between unirradiated and irradiated Pleurotus citrinopileatus. DNA polymorphism was studied for both irradiated P. citrinopileatus exposed to 1KGy and 2 KGy gamma rays.

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