Comparative analysis of whole cell proteins of Rhodococcus equi isolates using SDS-page

Author(s): Khurana S. K., Kanu Priya*, Namita Singh, H. Singha, Sarika Punia

Abstract

In the present study, the antigenic profile of four strains of R.equi isolated from nasal swabs of foals suffering from respiratory problems from different locations in Haryana was studied using SDS-PAGE in comparison to a standard strain collected from MTCC. This revealed that strain NS25 had 04 additional bands and 02 bands missing in comparison to standard strain. Strain NS44 had 07 additional and 02 missing bands. Strain NS48 had 07 additional and 07 missing bands whereas strain NS79 revealed 06 additional and 03 missing bands in comparison to standard strain. This showed that all the strains originating from varied locations were exhibiting different protein banding patterns thus varied on molecular epidemiological basis.

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