ASSAY OF IN VITRO FREE RADICAL SCAVENGERS AND THEIR ANTIOXIDANT ACTIVITY OF SORGHUM (SORGHUM BICOLOR (L.) MOENCH)

Author(s): Bangaru Naidu Thaddi* and Sarada Mani Nallamilli

Abstract

Sorghum is a one of the leading cereal crop, among the cereals Sorghum contains unique variety of bioactive compounds which affect the classification, colour, appearance, nutrition quality, and functionality of the grain. The present study deals with six different genotypes of Sorghum including three without pigmented testa (IS 3477, IS 33095, IS 7005), three pigmented testa (IS 7155, IS 2898, IS 1202) for their evolution of bioactive components and in vitro free radical scavenging of antioxidant activity. After the process of soxhlation, an obtained methanolic extract from the six genotypes of Sorghum, they were analyzed for their total phenolics and capacity of in vitro free radical assay included Superoxide radical, 1, 1-Diphenyl -2- pycrilhydrazyl (DPPH), Hydroxyl radical, and Lipid peroxidation. Here we have estimated the free radical scavenger’s order an increased level concentrations of percentage inhibition (10-1280 μg/ mL). The maximum IC50 values were favoured to pigmented testa genotypes of Sorghum. These various antioxidant activities were compared to excellent standard antioxidant like Ascorbic acid. The scavenging effects of all grain extracts on the DPPH radical were greater than that of compared with other free radicals. The pigmented testa genotypes exhibited the highest antioxidant capacities than non-pigmented testa genotypes and thus could be potential rich sources of natural antioxidants.

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